CDC50/YCR094W Summary Help

Standard Name CDC50 1, 2
Systematic Name YCR094W
Feature Type ORF, Verified
Description Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Cell Division Cycle 1, 2
Chromosomal Location
ChrIII:286762 to 287937 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CDC50 GO evidence and references
  View Computational GO annotations for CDC50
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Resources
144 total interaction(s) for 114 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • PCA: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 11
  • Negative Genetic: 68
  • Phenotypic Suppression: 3
  • Positive Genetic: 5
  • Synthetic Growth Defect: 19
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 21

Resources
Expression Summary
histogram
Resources
Length (a.a.) 391
Molecular Weight (Da) 44,982
Isoelectric Point (pI) 7.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:286762 to 287937 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1176 286762..287937 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000690
References cited on this page View Complete Literature Guide for CDC50
1) Moir D, et al.  (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
2) Radji M, et al.  (2001) The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae. Yeast 18(3):195-205
3) Misu K, et al.  (2003) Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae. Mol Biol Cell 14(2):730-47
4) Saito K, et al.  (2004) Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Mol Biol Cell 15(7):3418-32
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Lenoir G, et al.  (2009) Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter drs2p. J Biol Chem 284(27):17956-67