MSH3/YCR092C Summary Help

Standard Name MSH3
Systematic Name YCR092C
Feature Type ORF, Verified
Description Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability (1, 2 and see Summary Paragraph)
Name Description MutS Homolog
Chromosomal Location
ChrIII:279820 to 276764 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MSH3 GO evidence and references
  View Computational GO annotations for MSH3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
70 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 2
  • Co-purification: 2
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 8
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 1,018
Molecular Weight (Da) 116,533
Isoelectric Point (pI) 7.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:279820 to 276764 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2007-09-06
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3057 279820..276764 2011-02-03 2007-09-06
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000688

Msh3p functions in conjunction with Msh2p and Msh6p in the mismatch repair (MMR) system, which plays an important role in repairing single-base mismatches and mispaired regions due to small insertions or deletions (3). Genetic and biochemical results suggest that the Msh2p-Msh3p heterodimer has a different substrate specificity than the Msh2p-Msh6p heterodimer (4, 5, 6). The Msh2p-Msh3p heterodimer has a higher affinity for insertion-deletion loops larger than a single nucleotide and does not bind single-base mismatches with high affinity (5, 6, 7).

In addition to their role in mismatch repair, MSH2 and MSH3 are required for the removal of non-homologous DNA and regulation of heteroduplex formation during mitotic and meiotic recombination (1, 8, 9, 10). The Msh2p-Msh3p heterodimer binds double-strand/single-strand DNA junctions, possibly acting as a scaffold to recruit additional DNA repair proteins such as Mlh1p-Pms1p heterodimer or Rad1p-Rad10p heterodimer (11, 6).

Msh3p is one of six E. coli MutS homologs in S. cerevisiae but is more similar to the mouse Rep-3 protein (12). Mutations in the H. sapiens MSH3 protein have been identified in an endometrial carcinoma (OMIM) and in an endometrial carcinoma cell line (13).

Last updated: 2006-09-05 Contact SGD

References cited on this page View Complete Literature Guide for MSH3
1) Sugawara N, et al.  (1997) Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination. Proc Natl Acad Sci U S A 94(17):9214-9
2) Clark AB, et al.  (2000) Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes. J Biol Chem 275(47):36498-501
3) Harfe BD and Jinks-Robertson S  (2000) Mismatch repair proteins and mitotic genome stability. Mutat Res 451(1-2):151-67
4) Johnson RE, et al.  (1996) Requirement of the yeast MSH3 and MSH6 genes for MSH2-dependent genomic stability. J Biol Chem 271(13):7285-8
5) Alani E  (1996) The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs. Mol Cell Biol 16(10):5604-15
6) Surtees JA and Alani E  (2006) Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination. J Mol Biol 360(3):523-36
7) Habraken Y, et al.  (1996) Binding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair proteins MSH2 and MSH3. Curr Biol 6(9):1185-7
8) Studamire B, et al.  (1999) Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination. Mol Cell Biol 19(11):7558-67
9) Chen W and Jinks-Robertson S  (1998) Mismatch repair proteins regulate heteroduplex formation during mitotic recombination in yeast. Mol Cell Biol 18(11):6525-37
10) Stone JE and Petes TD  (2006) Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae. Genetics 173(3):1223-39
11) Habraken Y, et al.  (1997) Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex. Curr Biol 7(10):790-3
12) New L, et al.  (1993) The yeast gene MSH3 defines a new class of eukaryotic MutS homologues. Mol Gen Genet 239(1-2):97-108
13) Risinger JI, et al.  (1996) Mutation of MSH3 in endometrial cancer and evidence for its functional role in heteroduplex repair. Nat Genet 14(1):102-5