SRB8/YCR081W Summary Help

Standard Name SRB8 1
Systematic Name YCR081W
Alias GIG1 2 , NUT6 3 , SSN5 , YCR080W , MED12 4
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression (1, 2, 5)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrIII:254371 to 258654 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SRB8 GO evidence and references
  View Computational GO annotations for SRB8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 29 genes
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
195 total interaction(s) for 146 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 45
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Co-purification: 1
  • PCA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 56
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 37
  • Positive Genetic: 20
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 3
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,427
Molecular Weight (Da) 166,859
Isoelectric Point (pI) 6.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:254371 to 258654 | ORF Map | GBrowse
This feature contains embedded feature(s): YCR081C-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4284 254371..258654 2011-02-03 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000677
References cited on this page View Complete Literature Guide for SRB8
1) Hengartner CJ, et al.  (1995) Association of an activator with an RNA polymerase II holoenzyme. Genes Dev 9(8):897-910
2) Balciunas D and Ronne H  (1995) Three subunits of the RNA polymerase II mediator complex are involved in glucose repression. Nucleic Acids Res 23(21):4421-5
3) Tabtiang RK and Herskowitz I  (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
4) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
5) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9