BUD31/YCR063W Summary Help

Standard Name BUD31 1
Systematic Name YCR063W
Alias CWC14 2
Feature Type ORF, Verified
Description Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis (1, 3, 4, 5, 6)
Name Description BUD site selection 1
Chromosomal Location
ChrIII:228318 to 228791 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BUD31 GO evidence and references
  View Computational GO annotations for BUD31
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
210 total interaction(s) for 189 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 41
  • Affinity Capture-RNA: 2
  • Co-purification: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 116
  • Positive Genetic: 38
  • Synthetic Growth Defect: 6
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 157
Molecular Weight (Da) 18,447
Isoelectric Point (pI) 10.19
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:228318 to 228791 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..474 228318..228791 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000659
References cited on this page View Complete Literature Guide for BUD31
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Ohi MD, et al.  (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24
3) Troyanskaya OG, et al.  (2003) A Bayesian framework for combining heterogeneous data sources for gene function prediction (in Saccharomyces cerevisiae). Proc Natl Acad Sci U S A 100(14):8348-53
4) Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54
5) Saha D, et al.  (2012) Saccharomyces cerevisiae NineTeen complex (NTC)-associated factor Bud31/Ycr063w assembles on precatalytic spliceosomes and improves first and second step pre-mRNA splicing efficiency. J Biol Chem 287(8):5390-9
6) Saha D, et al.  (2012) Context dependent splicing functions of Bud31/Ycr063w define its role in budding and cell cycle progression. Biochem Biophys Res Commun 424(3):579-85