PER1/YCR044C Summary

PER1/YCR044C Summary

Standard Name	PER1
Systematic Name	YCR044C
Alias	COS16 1
Feature Type	ORF, Verified
Description	Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 (2)
Name Description	protein Processing in the ER 3

Chromosomal Location	ChrIII:207950 to 206877 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PER1 GO evidence and references

	View Computational GO annotations for PER1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	cellular manganese ion homeostasis (IMP) GPI anchor biosynthetic process (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) fungal-type vacuole membrane (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All PER1 Phenotype evidence and references

Classical genetics
null	heat sensitivity: increased Gas1p modification: decreased resistance to Calcofluor White: decreased resistance to staurosporine: decreased resistance to tunicamycin: decreased
reduction of function	heat sensitivity: increased resistance to Calcofluor White: decreased
Large-scale survey
null	budding pattern: abnormal cell shape: abnormal rubidium(1+) accumulation: decreased glycogen accumulation: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased haploinsufficient heat sensitivity: increased ionic stress resistance: decreased oxidative stress resistance: decreased protein transport: abnormal Can1p-GFP distribution: abnormal resistance to hygromycin B: decreased resistance to hydrogen chloride: decreased resistance to tunicamycin: decreased resistance to sulfometuron methyl: decreased resistance to sirolimus: decreased resistance to fenpropimorph: increased resistance to calcium dichloride: decreased resistance to caffeine: decreased resistance to 2,4-dichlorophenol: decreased resistance to bleomycin: decreased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to fluconazole: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to mycophenolic acid: decreased resistance to chloroquine: decreased resistance to mefloquine: decreased resistance to wortmannin: normal resistance to L-1,4-dithiothreitol: decreased resistance to 4-chlorophenol: decreased resistance to sorbate: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate stress resistance: decreased toxin resistance: decreased vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PER1 Interaction evidence and references

	313 total interaction(s) for 189 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 2 PCA: 2
Genetic Interactions	Negative Genetic: 161 Phenotypic Enhancement: 5 Phenotypic Suppression: 72 Positive Genetic: 34 Synthetic Growth Defect: 22 Synthetic Lethality: 3 Synthetic Rescue: 7
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PER1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIII:207950 to 206877 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1074	207950..206877	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000640

References cited on this page View Complete Literature Guide for PER1

1)	Paidhungat M and Garrett S (1998) Cdc1 and the vacuole coordinately regulate Mn2+ homeostasis in the yeast Saccharomyces cerevisiae. Genetics 148(4):1787-98
2)	Fujita M, et al. (2006) PER1 Is Required for GPI-Phospholipase A2 Activity and Involved in Lipid Remodeling of GPI-anchored Proteins. Mol Biol Cell 17(12):5253-64
3)	Ng DT, et al. (2000) The unfolded protein response regulates multiple aspects of secretory and membrane protein biogenesis and endoplasmic reticulum quality control. J Cell Biol 150(1):77-88