MATALPHA1/YCR040W Summary Help

Standard Name MATALPHA1 1
Systematic Name YCR040W
Alias ALPHA1
Feature Type ORF, Verified
Description Transcriptional co-activator that regulates mating-type-specific genes; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette (2, 3 and see Summary Paragraph)
Name Description MATing type protein ALPHA
Chromosomal Location
ChrIII:200442 to 200969 | ORF Map | GBrowse
Gene Ontology Annotations All MATALPHA1 GO evidence and references
  View Computational GO annotations for MATALPHA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
5 total interaction(s) for 5 unique genes/features.
Physical Interactions
  • Affinity Capture-Western: 1
  • PCA: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Suppression: 1

Expression Summary
Length (a.a.) 175
Molecular Weight (Da) 20,413
Isoelectric Point (pI) 10.83
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:200442 to 200969 | ORF Map | GBrowse
This feature is contained within: MATALPHA
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..528 200442..200969 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000636

alpha1 is one of two genes encoded by the alpha mating type cassette in S. cerevisiae. The mating type of S. cerevisiae is determined by the genetic composition of the MAT locus (4, 2, 5). Haploids of the a mating type express the genes a1 and a2 from the MAT locus while haploids of the alpha mating type express alpha1 and alpha2 from MAT (4, 2, 5). Some of the cells within a given colony are capable of switching mating type by changing the genetic composition of the MAT locus (6); more information on mating type switching can be found in the summary paragraph for MAT and references cited therein. Diploid cells are normally heterozygous (a/alpha) at the MAT locus (4, 2, 5).

alpha1 encodes a transcriptional co-activator involved in regulation of mating-type-specific gene expression (2, 5). alpha1p and the transcription factor Mcm1p bind cooperatively to sites in the promoters of alpha-specific genes; the role of alpha1p is to target Mcm1p to the promoters (3, 7, 8, 5).

Note: The maps and sequences within SGD reflect the fact that a MATalpha strain was used in the systematic sequencing project. Accordingly, there are two copies of the alpha1 and alpha2 genes; one pair (MATalpha1/YCR040W and MATalpha2/YCR039C) corresponds to the MAT locus, and the other (HMLalpha1/YCL066W and HMLalpha2/YCL067C) to the HML locus. There are ORFs assigned to the copies of a1 and a2 found at HMR (HMRa1/YCR097W) and (HMRa2/YCR096C); locus entries are included for MATa1 and MATa2 but they do not have ORFs associated with them. There are GenBank sequences available from all of the locus pages describing a1, a2, alpha1, and alpha2; the Genbank accession for the MATa cassette is V01313.

Last updated: 2000-08-17 Contact SGD

References cited on this page View Complete Literature Guide for MATALPHA1
1) Nasmyth KA, et al.  (1981) A position effect in the control of transcription at yeast mating type loci. Nature 289(5795):244-50
2) Herskowitz I  (1989) A regulatory hierarchy for cell specialization in yeast. Nature 342(6251):749-57
3) Bender A and Sprague GF Jr  (1987) MAT alpha 1 protein, a yeast transcription activator, binds synergistically with a second protein to a set of cell-type-specific genes. Cell 50(5):681-91
4) Herskowitz I  (1983) Cellular differentiation, cell lineages, and transposable genetic cassettes in yeast. Curr Top Dev Biol 18:1-14
5) Herskowitz I, et al.  (1992) "Mating-type determination and mating-type interconversion in Saccharomyces cerevisiae." Pp. 583-656 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Haber JE  (1998) Mating-type gene switching in Saccharomyces cerevisiae. Annu Rev Genet 32:561-99
7) Tan S, et al.  (1988) Interactions of purified transcription factors: binding of yeast MAT alpha 1 and PRTF to cell type-specific, upstream activating sequences. EMBO J 7(13):4255-64
8) Grayhack EJ  (1992) The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site. Mol Cell Biol 12(8):3573-82