RBK1/YCR036W Summary Help

Standard Name RBK1 1
Systematic Name YCR036W
Feature Type ORF, Verified
Description Putative ribokinase (1 and see Summary Paragraph)
Name Description RiBoKinase 1
Chromosomal Location
ChrIII:193297 to 194298 | ORF Map | GBrowse
Gbrowse
Genetic position: 29 cM
Gene Ontology Annotations All RBK1 GO evidence and references
  View Computational GO annotations for RBK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Large-scale survey
null
Resources
87 total interaction(s) for 82 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 57
  • Positive Genetic: 22
  • Synthetic Growth Defect: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 333
Molecular Weight (Da) 36,924
Isoelectric Point (pI) 5.04
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:193297 to 194298 | ORF Map | GBrowse
SGD ORF map
Genetic position: 29 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1002 193297..194298 2011-02-03 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000632
SUMMARY PARAGRAPH for RBK1

RBK1 encodes a putative ribokinase (EC: 2.7.1.15) predicted to be involved in ribose metabolism (1). Rbk1p has two regions of similarity to human ADK (OMIM), E. coli ribokinase, and the fructokinases from Kiebsiella, Salmonella, Vibrio, Rhizobium and potato, and has one region of similarity to E. coli inosine kinase and deoxygluconokinase (2, 1). Rbk1p localizes to the nucleus and cytoplasm (3).

rbk1 null mutants are viable and exhibit increased glycogen accumulation, as determined by iodine staining (4, 5). An active autonomously replicating sequence, ARS313, is located in the vicinity of RBK1 and hinders the isolation of an integrative disruption mutant (1).

Last updated: 2005-12-13 Contact SGD

References cited on this page View Complete Literature Guide for RBK1
1) Thierry A, et al.  (1990) The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase. Yeast 6(6):521-34
2) Spychala J, et al.  (1996) Cloning of human adenosine kinase cDNA: sequence similarity to microbial ribokinases and fructokinases. Proc Natl Acad Sci U S A 93(3):1232-7
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
5) Wilson WA, et al.  (2002) Systematic identification of the genes affecting glycogen storage in the yeast Saccharomyces cerevisiae: implication of the vacuole as a determinant of glycogen level. Mol Cell Proteomics 1(3):232-42