RBK1/YCR036W Summary

Standard Name	RBK1
Systematic Name	YCR036W
Feature Type	ORF, Verified
Description	Putative ribokinase (1 and see Summary Paragraph)
Name Description	RiBoKinase 1

Chromosomal Location	ChrIII:193297 to 194298 \| ORF Map \| GBrowse

	Genetic position: 29 cM

Gene Ontology Annotations All RBK1 GO evidence and references

	View Computational GO annotations for RBK1
Molecular Function
Manually curated	ATP binding (ISS) ribokinase activity (ISS)
Biological Process
Manually curated	D-ribose metabolic process (ISS)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All RBK1 Phenotype evidence and references

Large-scale survey
null	glycogen accumulation: increased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased hyperosmotic stress resistance: decreased resistance to ethanol: decreased resistance to arsenite(3-): decreased resistance to sodium selenide: decreased resistance to nickel(2+): increased resistance to L-1,4-dithiothreitol: decreased resistance to St. John's Wort extract: decreased stress resistance: decreased toxin resistance: increased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RBK1 Interaction evidence and references

	87 total interaction(s) for 82 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 57 Positive Genetic: 22 Synthetic Growth Defect: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RBK1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIII:193297 to 194298 | |

: 29 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 2000-09-13

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1002	193297..194298	2011-02-03	2000-09-13

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000632

SUMMARY PARAGRAPH for RBK1

RBK1 encodes a putative ribokinase (EC: 2.7.1.15) predicted to be involved in ribose metabolism (1). Rbk1p has two regions of similarity to human ADK (OMIM), E. coli ribokinase, and the fructokinases from Kiebsiella, Salmonella, Vibrio, Rhizobium and potato, and has one region of similarity to E. coli inosine kinase and deoxygluconokinase (2, 1). Rbk1p localizes to the nucleus and cytoplasm (3).

rbk1 null mutants are viable and exhibit increased glycogen accumulation, as determined by iodine staining (4, 5). An active autonomously replicating sequence, ARS313, is located in the vicinity of RBK1 and hinders the isolation of an integrative disruption mutant (1).

Last updated: 2005-12-13

References cited on this page View Complete Literature Guide for RBK1

1)	Thierry A, et al. (1990) The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase. Yeast 6(6):521-34
2)	Spychala J, et al. (1996) Cloning of human adenosine kinase cDNA: sequence similarity to microbial ribokinases and fructokinases. Proc Natl Acad Sci U S A 93(3):1232-7
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
5)	Wilson WA, et al. (2002) Systematic identification of the genes affecting glycogen storage in the yeast Saccharomyces cerevisiae: implication of the vacuole as a determinant of glycogen level. Mol Cell Proteomics 1(3):232-42