CIT2/YCR005C Summary Help

Standard Name CIT2 1
Systematic Name YCR005C
Feature Type ORF, Verified
Description Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description CITrate synthase 1
Chromosomal Location
ChrIII:122328 to 120946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CIT2 GO evidence and references
  View Computational GO annotations for CIT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 27 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
Resources
73 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • PCA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 23
  • Phenotypic Enhancement: 6
  • Positive Genetic: 8
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 460
Molecular Weight (Da) 51,413
Isoelectric Point (pI) 6.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:122328 to 120946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1383 122328..120946 2011-02-03 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000598
References cited on this page View Complete Literature Guide for CIT2
1) Kim KS, et al.  (1986) Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol Cell Biol 6(6):1936-42
2) Liao X and Butow RA  (1993) RTG1 and RTG2: two yeast genes required for a novel path of communication from mitochondria to the nucleus. Cell 72(1):61-71
3) Lewin AS, et al.  (1990) Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal. Mol Cell Biol 10(4):1399-405
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61