NFS1/YCL017C Summary Help

Standard Name NFS1 1
Systematic Name YCL017C
Alias SPL1
Feature Type ORF, Verified
Description Cysteine desulfurase; involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria (2, 3, 4)
Name Description NiFS-like 1
Chromosomal Location
ChrIII:94270 to 92777 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NFS1 GO evidence and references
  View Computational GO annotations for NFS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
unspecified
Large-scale survey
null
reduction of function
Resources
33 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 2
  • Co-fractionation: 3
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 497
Molecular Weight (Da) 54,467
Isoelectric Point (pI) 8.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:94270 to 92777 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-12 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1494 94270..92777 2006-01-12 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000522
References cited on this page View Complete Literature Guide for NFS1
1) Kolman C and Soll D  (1993) SPL1-1, a Saccharomyces cerevisiae mutation affecting tRNA splicing. J Bacteriol 175(5):1433-42
2) Kispal G, et al.  (1999) The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins. EMBO J 18(14):3981-9
3) Nakai Y, et al.  (2004) Yeast Nfs1p is involved in thio-modification of both mitochondrial and cytoplasmic tRNAs. J Biol Chem 279(13):12363-8
4) Muhlenhoff U, et al.  (2004) Functional characterization of the eukaryotic cysteine desulfurase Nfs1p from Saccharomyces cerevisiae. J Biol Chem 279(35):36906-15