YCK3/YER123W Summary Help

Standard Name YCK3 1
Systematic Name YER123W
Alias CKI3
Feature Type ORF, Verified
Description Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway (1, 2, 3, 4)
Name Description Yeast Casein Kinase 1
Chromosomal Location
ChrV:404813 to 406387 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YCK3 GO evidence and references
  View Computational GO annotations for YCK3
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
102 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 5
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 4

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 1
  • Negative Genetic: 57
  • Phenotypic Enhancement: 3
  • Positive Genetic: 10
  • Synthetic Growth Defect: 6
  • Synthetic Haploinsufficiency: 1
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 524
Molecular Weight (Da) 60,260
Isoelectric Point (pI) 9.08
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:404813 to 406387 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2000-03-16
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1575 404813..406387 2011-02-03 2000-03-16
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000925
References cited on this page View Complete Literature Guide for YCK3
1) Wang X, et al.  (1996) Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase. Mol Cell Biol 16(10):5375-85
2) Sun B, et al.  (2004) The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal. Mol Biol Cell 15(3):1397-406
3) Lagrassa TJ and Ungermann C  (2005) The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex. J Cell Biol 168(3):401-14
4) Anand VC, et al.  (2009) Genome-wide analysis of AP-3-dependent protein transport in yeast. Mol Biol Cell 20(5):1592-604