YCK2/YNL154C Summary Help

Standard Name YCK2 1
Systematic Name YNL154C
Feature Type ORF, Verified
Description Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK2 has a paralog, YCK1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description Yeast Casein Kinase 1
Chromosomal Location
ChrXIV:345276 to 343636 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All YCK2 GO evidence and references
  View Computational GO annotations for YCK2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 58 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
389 total interaction(s) for 352 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 224
  • Co-fractionation: 1
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 1
  • Negative Genetic: 94
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 14
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 9
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 546
Molecular Weight (Da) 62,079
Isoelectric Point (pI) 9.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:345276 to 343636 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1641 345276..343636 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005098
References cited on this page View Complete Literature Guide for YCK2
1) Robinson LC, et al.  (1992) Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A 89(1):28-32
2) Marchal C, et al.  (2000) Casein kinase I-dependent phosphorylation within a PEST sequence and ubiquitination at nearby lysines signal endocytosis of yeast uracil permease. J Biol Chem 275(31):23608-14
3) Hicke L, et al.  (1998) Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization. J Cell Biol 141(2):349-58
4) Wang PC, et al.  (1992) Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell 3(3):275-86
5) Robinson LC, et al.  (1993) Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis. Mol Cell Biol 13(5):2870-81
6) Robinson LC, et al.  (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92
7) Feng Y and Davis NG  (2000) Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane. Mol Cell Biol 20(14):5350-9
8) Roth AF, et al.  (2002) The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J Cell Biol 159(1):23-8
9) Babu P, et al.  (2004) Akr1p-dependent palmitoylation of Yck2p yeast casein kinase 1 is necessary and sufficient for plasma membrane targeting. J Biol Chem 279(26):27138-47
10) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
11) Reddi AR and Culotta VC  (2013) SOD1 integrates signals from oxygen and glucose to repress respiration. Cell 152(1-2):224-35