YCK1/YHR135C Summary Help

Standard Name YCK1 1
Systematic Name YHR135C
Alias CKI2
Feature Type ORF, Verified
Description Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK1 has a paralog, YCK2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Yeast Casein Kinase I homologue 1
Chromosomal Location
ChrVIII:374310 to 372694 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All YCK1 GO evidence and references
  View Computational GO annotations for YCK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
457 total interaction(s) for 402 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 58
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 292
  • PCA: 2
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 44
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 6
  • Positive Genetic: 4
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 11
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 538
Molecular Weight (Da) 61,715
Isoelectric Point (pI) 9.81
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:374310 to 372694 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1617 374310..372694 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001177
References cited on this page View Complete Literature Guide for YCK1
1) Robinson LC, et al.  (1992) Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A 89(1):28-32
2) Marchal C, et al.  (2000) Casein kinase I-dependent phosphorylation within a PEST sequence and ubiquitination at nearby lysines signal endocytosis of yeast uracil permease. J Biol Chem 275(31):23608-14
3) Hicke L, et al.  (1998) Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization. J Cell Biol 141(2):349-58
4) Wang PC, et al.  (1992) Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell 3(3):275-86
5) Robinson LC, et al.  (1993) Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis. Mol Cell Biol 13(5):2870-81
6) Robinson LC, et al.  (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92
7) Feng Y and Davis NG  (2000) Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane. Mol Cell Biol 20(14):5350-9
8) Roth AF, et al.  (2002) The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J Cell Biol 159(1):23-8
9) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
10) Reddi AR and Culotta VC  (2013) SOD1 integrates signals from oxygen and glucose to repress respiration. Cell 152(1-2):224-35