YCF1/YDR135C Summary Help

Standard Name YCF1 1
Systematic Name YDR135C
Feature Type ORF, Verified
Description Vacuolar glutathione S-conjugate transporter; member of ATP-binding cassette family; ATPase activity required to support vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; has role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR (1, 2, 3, 4, 5, 6, 7)
Name Description Yeast Cadmium Factor 1
Chromosomal Location
ChrIV:727551 to 723004 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All YCF1 GO evidence and references
  View Computational GO annotations for YCF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
activation
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
93 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • FRET: 1
  • PCA: 38
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 13
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 6
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,515
Molecular Weight (Da) 171,120
Isoelectric Point (pI) 8.49
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:727551 to 723004 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4548 727551..723004 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002542
References cited on this page View Complete Literature Guide for YCF1
1) Szczypka MS, et al.  (1994) A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein. J Biol Chem 269(36):22853-7
2) Petrovic S, et al.  (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71
3) Li ZS, et al.  (1996) The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump. J Biol Chem 271(11):6509-17
4) Li ZS, et al.  (1997) A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium. Proc Natl Acad Sci U S A 94(1):42-7
5) Gueldry O, et al.  (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96
6) Lazard M, et al.  (2011) Selenodiglutathione uptake by the Saccharomyces cerevisiae vacuolar ATP-binding cassette transporter Ycf1p. FEBS J 278(21):4112-21
7) Sasser TL, et al.  (2013) The Yeast ATP-binding Cassette (ABC) Transporter Ycf1p Enhances the Recruitment of the Soluble SNARE Vam7p to Vacuoles for Efficient Membrane Fusion. J Biol Chem 288(25):18300-10