YCF1/YDR135C Summary

YCF1 BASIC INFORMATION

Standard Name	YCF1
Systematic Name	YDR135C
Feature Type	ORF, Verified
Description	Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin; similar to human cystic fibrosis protein CFTR (1, 2, 3, 4, 5)
Name Description	Yeast Cadmium Factor 4

GO Annotations	All YCF1 GO evidence and references
	View Computational GO annotations for YCF1
Molecular Function
Manually curated	bilirubin transmembrane transporter activity (IGI, IMP) glutathione S-conjugate-exporting ATPase activity (IDA)
Biological Process
Manually curated	bilirubin transport (IGI, IMP) response to metal ion (IDA, TAS) transmembrane transport (IMP)
High-throughput	response to drug (IMP)
Cellular Component
Manually curated	vacuolar membrane (IDA)

Mutant Phenotype	All YCF1 Phenotype details and references
Classical genetics
null	cell size: increased metal resistance: increased metal resistance: decreased oxidative stress resistance: decreased
overexpression	resistance to diazaborine: increased
Large-scale survey
null	budding index: increased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to cadmium dichloride: decreased viable

Interactions	YCF1 All interactions details and references
	25 total interaction(s) for 18 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Two-hybrid: 11
Genetic Interactions	Dosage Rescue: 2 Phenotypic Enhancement: 6 Synthetic Growth Defect: 2

Sequence Information

ChrIV:727549 to 723002 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4548	727549..723002	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| TCDB \| UniProtKB

Primary SGDID	S000002542

YCF1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for YCF1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for YCF1]

1)	Petrovic S, et al. (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71
2)	Li ZS, et al. (1996) The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump. J Biol Chem 271(11):6509-17
3)	Li ZS, et al. (1997) A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium. Proc Natl Acad Sci U S A 94(1):42-7
4)	Szczypka MS, et al. (1994) A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein. J Biol Chem 269(36):22853-7
5)	Gueldry O, et al. (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96

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