DPB3/YBR278W Summary Help

Standard Name DPB3 1
Systematic Name YBR278W
Feature Type ORF, Verified
Description Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description DNA Polymerase B (II) subunit 1
Chromosomal Location
ChrII:760294 to 760899 | ORF Map | GBrowse
Gene Ontology Annotations All DPB3 GO evidence and references
  View Computational GO annotations for DPB3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
290 total interaction(s) for 179 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 7
  • Co-fractionation: 1
  • Co-purification: 4
  • Reconstituted Complex: 4
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 167
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 8
  • Positive Genetic: 30
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 5
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 201
Molecular Weight (Da) 22,665
Isoelectric Point (pI) 4.35
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:760294 to 760899 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..606 760294..760899 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000482
References cited on this page View Complete Literature Guide for DPB3
1) Araki H, et al.  (1991) Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae. Nucleic Acids Res 19(18):4867-72
2) Li Y, et al.  (2000) Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon. J Biol Chem 275(30):23247-52
3) Iida T and Araki H  (2004) Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae. Mol Cell Biol 24(1):217-27
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Aksenova A, et al.  (2010) Mismatch Repair-Independent Increase in Spontaneous Mutagenesis in Yeast Lacking Non-Essential Subunits of DNA Polymerase epsilon. PLoS Genet 6(11):e1001209