PDB1/YBR221C Summary Help

Standard Name PDB1 1
Systematic Name YBR221C
Feature Type ORF, Verified
Description E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria (2 and see Summary Paragraph)
Name Description Pyruvate Dehydrogenase Beta subunit 2
Chromosomal Location
ChrII:666253 to 665153 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PDB1 GO evidence and references
  View Computational GO annotations for PDB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
Resources
204 total interaction(s) for 160 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 53
  • Affinity Capture-RNA: 4
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 103
  • Positive Genetic: 31
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 366
Molecular Weight (Da) 40,053
Isoelectric Point (pI) 5.06
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:666253 to 665153 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1101 666253..665153 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000425
SUMMARY PARAGRAPH for PDB1

PDB1 encodes the beta subunit of pyruvate dehydrogenase (3, 4). Pyruvate dehydrogenase, consisting of alpha (Pda1p) and beta (Pdb1p) subunits, is the E1 component of the large multienzyme pyruvate dehydrogenase complex (5). This mitochondrial complex consists of an E2 (Lat1p) core that binds E1 (Pda1p and Pdb1p) , E3 (Lpd1p), and Protein X (Pdx1p) components (5). E1 (pyruvate dehydrogenase), E2 (dihydrolipoamide acetyltransferase), and E3 (dihydrolipoamide dehydrogenase) act in concert to convert pyruvate into acetyl-CoA (5). Cells lacking PDB1 are viable but lack pyruvate dehydrogenase activity (2). The yeast PDB1 gene shows strong similarity to human PDHB, which encodes the human E1 beta sub unit (2).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for PDB1
1) Steensma, H.Y.  (1992) Personal Communication, Mortimer Map Edition 11
2) Miran SG, et al.  (1993) Characterization of PDH beta 1, the structural gene for the pyruvate dehydrogenase beta subunit from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 90(4):1252-6
3) Behal RH, et al.  (1989) Nucleotide and deduced amino acid sequence of the alpha subunit of yeast pyruvate dehydrogenase. Biochem Biophys Res Commun 164(2):941-6
4) Steensma HY, et al.  (1990) Molecular cloning of the gene for the E1 alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae. Eur J Biochem 191(3):769-74
5) Pronk JT, et al.  (1996) Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12(16):1607-33