NGR1/YBR212W Summary Help

Standard Name NGR1 1, 2
Systematic Name YBR212W
Alias RBP1 3
Feature Type ORF, Verified
Description RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase (3, 4, 5, 6, 7)
Name Description Negative Growth Regulatory protein
Chromosomal Location
ChrII:647886 to 649904 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NGR1 GO evidence and references
  View Computational GO annotations for NGR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 119 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
73 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 24
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 24
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 672
Molecular Weight (Da) 75,023
Isoelectric Point (pI) 8.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:647886 to 649904 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2019 647886..649904 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000416
References cited on this page View Complete Literature Guide for NGR1
1) Li AM, et al.  (2003) Scp160p associates with specific mRNAs in yeast. Nucleic Acids Res 31(7):1830-7
2) Gardner JM, et al.  (2005) Identification of genes affecting glucose catabolism in nitrogen-limited fermentation. FEMS Yeast Res 5(9):791-800
3) Lee FJ and Moss J  (1993) An RNA-binding protein gene (RBP1) of Saccharomyces cerevisiae encodes a putative glucose-repressible protein containing two RNA recognition motifs. J Biol Chem 268(20):15080-7
4) Akada R, et al.  (1997) Screening and identification of yeast sequences that cause growth inhibition when overexpressed. Mol Gen Genet 254(3):267-74
5) Buu LM, et al.  (2004) The yeast RNA-binding protein Rbp1p modifies the stability of mitochondrial porin mRNA. J Biol Chem 279(1):453-62
6) Martinez MJ, et al.  (2004) Genomic analysis of stationary-phase and exit in Saccharomyces cerevisiae: gene expression and identification of novel essential genes. Mol Biol Cell 15(12):5295-305
7) Chang LC and Lee FJ  (2012) The RNA helicase Dhh1p cooperates with Rbp1p to promote porin mRNA decay via its non-conserved C-terminal domain. Nucleic Acids Res 40(3):1331-44