AME1/YBR211C Summary Help

Standard Name AME1 1
Systematic Name YBR211C
Alias ARP100
Feature Type ORF, Verified
Description Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4)
Name Description Associated with Microtubules and Essential 1
Chromosomal Location
ChrII:647132 to 646158 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All AME1 GO evidence and references
  View Computational GO annotations for AME1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
conditional
null
reduction of function
Large-scale survey
conditional
null
overexpression
repressible
unspecified
Resources
192 total interaction(s) for 101 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 54
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • PCA: 4
  • Reconstituted Complex: 9
  • Two-hybrid: 35

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 48
  • Positive Genetic: 16
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 324
Molecular Weight (Da) 37,461
Isoelectric Point (pI) 4.57
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:647132 to 646158 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..975 647132..646158 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000415
References cited on this page View Complete Literature Guide for AME1
1) Pot I, et al.  (2005) Spindle checkpoint maintenance requires Ame1 and Okp1. Cell Cycle 4(10):1448-56
2) De Wulf P, et al.  (2003) Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev 17(23):2902-21
3) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76