MAK5/YBR142W Summary Help

Standard Name MAK5 1
Systematic Name YBR142W
Feature Type ORF, Verified
Description Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits (1, 2)
Name Description MAintenance of Killer 3
Chromosomal Location
ChrII:528317 to 530638 | ORF Map | GBrowse
Gbrowse
Genetic position: 80 cM
Gene Ontology Annotations All MAK5 GO evidence and references
  View Computational GO annotations for MAK5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
dominant negative
null
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
136 total interaction(s) for 90 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 106
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 8
  • Biochemical Activity: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 3
  • Phenotypic Suppression: 4
  • Positive Genetic: 2
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 773
Molecular Weight (Da) 87,047
Isoelectric Point (pI) 8.74
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:528317 to 530638 | ORF Map | GBrowse
SGD ORF map
Genetic position: 80 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2322 528317..530638 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000346
References cited on this page View Complete Literature Guide for MAK5
1) Zagulski M, et al.  (2003) Mak5p, which is required for the maintenance of the M1 dsRNA virus, is encoded by the yeast ORF YBR142w and is involved in the biogenesis of the 60S subunit of the ribosome. Mol Genet Genomics 270(3):216-24
2) Wickner RB and Leibowitz MJ  (1976) Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast. J Mol Biol 105(3):427-43
3) Wickner RB  (1974) Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics 76(3):423-32