EXO84/YBR102C Summary Help

Standard Name EXO84 1
Systematic Name YBR102C
Alias USA3 2
Feature Type ORF, Verified
Description Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate (1, 2, 3, 4)
Name Description EXOcyst 5
Chromosomal Location
ChrII:447323 to 445062 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All EXO84 GO evidence and references
  View Computational GO annotations for EXO84
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
null
overexpression
repressible
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
115 total interaction(s) for 58 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Biochemical Activity: 4
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 32

Genetic Interactions
  • Dosage Rescue: 9
  • Positive Genetic: 2
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 753
Molecular Weight (Da) 85,537
Isoelectric Point (pI) 8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:447323 to 445062 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2262 447323..445062 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000306
References cited on this page View Complete Literature Guide for EXO84
1) Guo W, et al.  (1999) Exo84p is an exocyst protein essential for secretion. J Biol Chem 274(33):23558-64
2) Awasthi S, et al.  (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15
3) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4) Zhang X, et al.  (2005) The critical role of Exo84p in the organization and polarized localization of the exocyst complex. J Biol Chem 280(21):20356-64
5) TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94