SFT2/YBL102W Summary

Standard Name	SFT2
Systematic Name	YBL102W
Feature Type	ORF, Verified
Description	Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 (1, 2, 3 and see Summary Paragraph)
Name Description	Suppressor of sedFive Ts 2

Chromosomal Location	ChrII:24098 to 24745 \| ORF Map \| GBrowse

	View Computational GO annotations for SFT2
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	Golgi to endosome transport (IGI, IMP)
Cellular Component
Manually curated	Golgi membrane (IDA)
High-throughput	integral to membrane (ISM)

Large-scale survey
null	auxotrophy competitive fitness: decreased viable
overexpression	inviable vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

	172 total interaction(s) for 95 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Co-localization: 1 PCA: 18 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 123 Positive Genetic: 17 Synthetic Growth Defect: 3 Synthetic Lethality: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:24098 to 24745 | |

Last Update

Coordinates: 2004-01-26 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..648	24098..24745	2004-01-26	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000198

SUMMARY PARAGRAPH for SFT2

Sft2p is a non-essential protein which colocalizes with Sft1p in the late Golgi cisternae, and interacts genetically with the early-Golgi "target" SNARE, Sed5p (2, 1). Sft2p has four predicted transmembrane domains, and can be used as a late Golgi marker to label Golgi membranes. Its N-terminus is predicted to be cytoplasmic (3). Sft2p appears to be involved in Golgi-to-endosome transport, but its precise function is currently undefined (1, 3).

Deletion of SFT2 is not normally lethal, but mutants have been isolated that require Sft2p for growth (3).

Last updated: 2005-06-07

1)	Conchon S, et al. (1999) Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex. EMBO J 18(14):3934-46
2)	Banfield DK, et al. (1995) A SNARE-like protein required for traffic through the Golgi complex. Nature 375(6534):806-9
3)	Wooding S and Pelham HR (1998) The dynamics of golgi protein traffic visualized in living yeast cells. Mol Biol Cell 9(9):2667-80