KTI11/YBL071W-A Summary Help

Standard Name KTI11 1, 2
Systematic Name YBL071W-A
Alias DPH3 3
Feature Type ORF, Verified
Description Zn-ribbon protein that co-purifies with Dph1 and Dph2; in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs (2, 4, 5, 6, 7, 8)
Name Description Kluveromyces lactis Toxin Insensitive 9
Chromosomal Location
ChrII:89978 to 90226 | ORF Map | GBrowse
Gene Ontology Annotations All KTI11 GO evidence and references
  View Computational GO annotations for KTI11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
dominant negative
reduction of function
Large-scale survey
44 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 15
  • Co-purification: 1
  • PCA: 5
  • Protein-RNA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 82
Molecular Weight (Da) 9,356
Isoelectric Point (pI) 3.56
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:89978 to 90226 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2001-01-30
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..249 89978..90226 2011-02-03 2001-01-30
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007587
References cited on this page View Complete Literature Guide for KTI11
1) Butler AR, et al.  (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
2) Fichtner L and Schaffrath R  (2002) KTI11 and KTI13, Saccharomyces cerevisiae genes controlling sensitivity to G1 arrest induced by Kluyveromyces lactis zymocin. Mol Microbiol 44(3):865-75
3) Chen JY, et al.  (1985) Diphtheria toxin-resistant mutants of Saccharomyces cerevisiae. Mol Cell Biol 5(12):3357-60
4) Fichtner L, et al.  (2003) Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification. Mol Microbiol 49(5):1297-307
5) Liu S, et al.  (2004) Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Mol Cell Biol 24(21):9487-97
6) Huang B, et al.  (2005) An early step in wobble uridine tRNA modification requires the Elongator complex. RNA 11(4):424-36
7) Proudfoot M, et al.  (2008) Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain. J Mol Biol 375(1):301-15
8) Bar C, et al.  (2008) A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3. Mol Microbiol 69(5):1221-33
9) Butler AR, et al.  (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25