AST1/YBL069W Summary Help

Standard Name AST1 1
Systematic Name YBL069W
Feature Type ORF, Verified
Description Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication (1, 2, 3, 4 and see Summary Paragraph)
Name Description ATPase STabilizing 1
Chromosomal Location
ChrII:90741 to 92030 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All AST1 GO evidence and references
  View Computational GO annotations for AST1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All AST1 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All AST1 Interaction evidence and references
11 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1
Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
Resources
Expression Summary
histogram
Resources
Protein Information All AST1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:90741 to 92030 | ORF Map | GBrowse
This feature contains embedded feature(s): YBL068W-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2001-05-29
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1290 90741..92030 2011-02-03 2001-05-29
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000165
SUMMARY PARAGRAPH for AST1

About the medium-chain dehydrogenase/reductase (MDR) family

Medium-chain dehydrogenase/reductases (MDRs), sometimes referred to as long-chain dehydrogenases (5), constitute an ancient and widespread enzyme superfamily with members found in Bacteria, Archaea, and Eukaryota (6, 4). Many MDR members are basic metabolic enzymes acting on alcohols or aldehydes, and thus these enzymes may have roles in detoxifying alcohols and related compounds, protecting against environmental stresses such as osmotic shock, reduced or elevated temperatures, or oxidative stress (6). The family also includes the mammalian zeta-crystallin lens protein, which may protect the lens against oxidative damage and enzymes which produce lignocellulose in plants (6).

MDR enzymes typically have subunits of about 350 aa residues and are two-domain proteins, with a catalytic domain and a second domain for binding to the nicotinamide cofactor, either NAD(H) or NADP(H) (6, 4). They contain 0, 1, or 2 zinc atoms (7). When zinc is present, it is involved in catalysis at the active site.

Based on phylogenetic and sequence analysis, the members of the MDR superfamily can be further divided into more closely related subgroups (6, 4). In families which are widespread from prokaryotes to eukaryotes, some members appear conserved across all species, while others appear to be due to lineage specific duplications. Some subgroups are only found in certain taxa. S. cerevisiae contains fifteen (6) or twenty-one (4) members of the MDR superfamily, listed below. The difference in number is due to six sequences that were included as members of the quinone oxidoreductase family by Riveros-Rosas et al. (4) but not by Nordling et al. (6).

Zinc-containing enzyme groups:
- PDH; "polyol" dehydrogenase family - BDH1, BDH2, SOR1, SOR2, XYL2
- ADH; class III alcohol dehydrogenase family - SFA1
- Y-ADH; "yeast" alcohol dehydrogenase family - ADH1, ADH2, ADH3, ADH5
- CADH; cinnamyl alcohol dehydrogenase family - ADH6, ADH7

Non-zinc-containing enzyme groups:
- NRBP; nuclear receptor binding protein (4) or MRF; mitochondrial respiratory function (6) family - ETR1
- QOR; quinone oxidoreductase family - ZTA1 (6, 4), AST1, AST2, YCR102C, YLR460C, YMR152W, YNL134C (4)
- LTD; leukotriene B4 dehydrogenases - YML131W
- ER; enoyl reductases (4) or ACR; acyl-CoA reductase (6) family - no members in S. cerevisiae

Last updated: 2008-08-19

References cited on this page View Complete Literature Guide for AST1
1) Chang A and Fink GR  (1995) Targeting of the yeast plasma membrane [H+]ATPase: a novel gene AST1 prevents mislocalization of mutant ATPase to the vacuole. J Cell Biol 128(1-2):39-49
2) Bagnat M, et al.  (2001) Plasma membrane proton ATPase Pma1p requires raft association for surface delivery in yeast. Mol Biol Cell 12(12):4129-38
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Riveros-Rosas H, et al.  (2003) Diversity, taxonomy and evolution of medium-chain dehydrogenase/reductase superfamily. Eur J Biochem 270(16):3309-34
5) Jornvall H, et al.  (1981) Alcohol and polyol dehydrogenases are both divided into two protein types, and structural properties cross-relate the different enzyme activities within each type. Proc Natl Acad Sci U S A 78(7):4226-30
6) Nordling E, et al.  (2002) Medium-chain dehydrogenases/reductases (MDR). Family characterizations including genome comparisons and active site modeling. Eur J Biochem 269(17):4267-76
7) Persson B, et al.  (1999) Bioinformatics in studies of SDR and MDR enzymes. Adv Exp Med Biol 463:373-7