FMT1/YBL013W Summary Help

Standard Name FMT1 1
Systematic Name YBL013W
Feature Type ORF, Verified
Description Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate (1, 2 and see Summary Paragraph)
Name Description Formyl-Methionyl-tRNA Transformylase
Gene Product Alias methionyl-tRNA transformylase 1
Chromosomal Location
ChrII:202056 to 203261 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All FMT1 GO evidence and references
  View Computational GO annotations for FMT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
unspecified
Resources
58 total interaction(s) for 55 unique genes/features.
Physical Interactions
  • Biochemical Activity: 1

Genetic Interactions
  • Negative Genetic: 38
  • Phenotypic Enhancement: 1
  • Positive Genetic: 17
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 401
Molecular Weight (Da) 44,616
Isoelectric Point (pI) 10.39
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:202056 to 203261 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2004-01-29
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1206 202056..203261 2011-02-03 2004-01-29
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000109
SUMMARY PARAGRAPH for FMT1

FMT1 encodes methionyl-tRNA formyltransferase, which catalyzes the formylation of initiator Met-tRNA (1). In yeast mitochondria, as in eubacteria and mitochondria from other eukaryotes, protein synthesis initiates with formylmethionine; eukaryotic cytosolic protein synthesis initiates with methionine (3). Although there is no formylation of the initiator Met-tRNA in cells lacking FMT1, the cells are viable and show normal mitochondrial protein synthesis and function (1).

Methionyl-tRNA formyltransferases have been identified in many prokaryotes and eukaryotes (1, 4). The structure of the E. coli enzyme has been determined (5, 6); a gene encoding mitochondrial methionyl-tRNA formyltransferase has been cloned from bovine liver (7).

Last updated: 2000-05-24 Contact SGD

References cited on this page View Complete Literature Guide for FMT1
1) Li Y, et al.  (2000) Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA. J Bacteriol 182(10):2886-92
2) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
3) Kozak M  (1983) Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev 47(1):1-45
4) Gite S, et al.  (2000) Escherichia coli methionyl-tRNA formyltransferase: role of amino acids conserved in the linker region and in the C-terminal domain on the specific recognition of the initiator tRNA. Biochemistry 39(9):2218-26
5) Schmitt E, et al.  (1996) Structure of crystalline Escherichia coli methionyl-tRNA(f)Met formyltransferase: comparison with glycinamide ribonucleotide formyltransferase. EMBO J 15(17):4749-58
6) Schmitt E, et al.  (1998) Crystal structure of methionyl-tRNAfMet transformylase complexed with the initiator formyl-methionyl-tRNAfMet. EMBO J 17(23):6819-26
7) Takeuchi N, et al.  (1998) Expression and characterization of bovine mitochondrial methionyl-tRNA transformylase. J Biochem 124(6):1069-71