BUD14 encodes a regulatory subunit for the type-1 protein phosphatase Glc7p, and is required for its localization to the bud cortex and shmoo tip (2). The Bud14p-Glc7p complex functions to stabilize microtubule interactions specifically at sites of polarized growth (2). The Bud14p-Glc7p complex also appears to play a role in the regulation of STRE-dependent transcription by cooperating with the Ccr4p-Not complex to regulate Msn2p activity (5). Bud14p has also been reported to be important for bud site selection (1).

BUD14 is not required for viability, but bud14 null mutants display increased sensitivity to mating factor, increased filamentous growth, hyperelongated shmoo morphology, and a mild stimulation of pheromone response pathway reporters as compared to wild type (6). bud14 null mutants also exhibit a defect in maintaining the position of the pre-anaphase spindle at the bud neck (2), and a random budding pattern with an elongated bud morphology (1). Overexpression of Bud14p is toxic and promotes the formation of long astral microtubules and increased dynein-dependent sliding along the cell cortex (2). Bud14p accumulates at sites of polarized growth and the mother-bud neck during cytokinesis in a manner that is dependent on an intact actin cytoskeleton and the kelch-domain-containing proteins Kel1p and Kel2p (2).

Last updated: 2005-09-26