BUD14/YAR014C Summary Help

Standard Name BUD14 1
Systematic Name YAR014C
Feature Type ORF, Verified
Description Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4 and see Summary Paragraph)
Name Description BUD site selection 1
Chromosomal Location
ChrI:168871 to 166742 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BUD14 GO evidence and references
  View Computational GO annotations for BUD14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
219 total interaction(s) for 170 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 4
  • Co-localization: 4
  • PCA: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 61

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 55
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 24
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 5
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 709
Molecular Weight (Da) 78,981
Isoelectric Point (pI) 4.1
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:168871 to 166742 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2130 168871..166742 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000069

BUD14 encodes a regulatory subunit for the type-1 protein phosphatase Glc7p, and is required for its localization to the bud cortex and shmoo tip (2). The Bud14p-Glc7p complex functions to stabilize microtubule interactions specifically at sites of polarized growth (2). The Bud14p-Glc7p complex also appears to play a role in the regulation of STRE-dependent transcription by cooperating with the Ccr4p-Not complex to regulate Msn2p activity (5). Bud14p has also been reported to be important for bud site selection (1).

BUD14 is not required for viability, but bud14 null mutants display increased sensitivity to mating factor, increased filamentous growth, hyperelongated shmoo morphology, and a mild stimulation of pheromone response pathway reporters as compared to wild type (6). bud14 null mutants also exhibit a defect in maintaining the position of the pre-anaphase spindle at the bud neck (2), and a random budding pattern with an elongated bud morphology (1). Overexpression of Bud14p is toxic and promotes the formation of long astral microtubules and increased dynein-dependent sliding along the cell cortex (2). Bud14p accumulates at sites of polarized growth and the mother-bud neck during cytokinesis in a manner that is dependent on an intact actin cytoskeleton and the kelch-domain-containing proteins Kel1p and Kel2p (2).

Last updated: 2005-09-26 Contact SGD

References cited on this page View Complete Literature Guide for BUD14
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Knaus M, et al.  (2005) The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast. EMBO J 24(17):3000-11
3) Chesarone M, et al.  (2009) Displacement of formins from growing barbed ends by bud14 is critical for actin cable architecture and function. Dev Cell 16(2):292-302
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Lenssen E, et al.  (2005) The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution. Mol Cell Biol 25(1):488-98
6) Cullen PJ and Sprague GF Jr  (2002) The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae. Eukaryot Cell 1(6):884-94