GDH3/YAL062W Summary Help

Standard Name GDH3
Systematic Name YAL062W
Alias FUN51
Feature Type ORF, Verified
Description NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication (1, 2, 3, 4 and see Summary Paragraph)
Name Description Glutamate DeHydrogenase
Chromosomal Location
ChrI:31567 to 32940 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GDH3 GO evidence and references
  View Computational GO annotations for GDH3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 11 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
unspecified
Resources
32 total interaction(s) for 23 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 7
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 457
Molecular Weight (Da) 49,627
Isoelectric Point (pI) 5.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:31567 to 32940 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1374 31567..32940 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000058
SUMMARY PARAGRAPH for GDH3

About glutamate biosynthesis from ammonia

Yeast cells contain 3 pathways for the synthesis of glutamate. Two pathways are mediated by two isoforms of glutamate dehydrogenase, encoded by GDH1 and GDH3 (5, 1). The third pathway is driven by the combined activities of glutamine synthetase and glutamate synthase, encoded by GLN1 and GLT1, respectively (6, 7). Studies of GDH1 and GDH3 regulation indicate that the cell uses these isoforms under different growth conditions (2). Expression of GDH3 is induced by ethanol and repressed by glucose, whereas GDH1 expression is high in either carbon source. Gdh1p uses alpha-ketoglutarate at a higher rate than Gdh3p. Thus, under fermentative growth conditions, Gdh1p drives glutamate biosynthesis, whereas in nonfermentable or limiting carbon sources, Gdh3p is the key isoform involved in balancing distribution of alpha-ketoglutarate to glutamate biosynthesis and energy metabolism.

Last updated: 2007-10-04 Contact SGD

References cited on this page View Complete Literature Guide for GDH3
1) Avendano A, et al.  (1997) GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. J Bacteriol 179(17):5594-7
2) DeLuna A, et al.  (2001) NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles. J Biol Chem 276(47):43775-83
3) Riego L, et al.  (2002) GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth. Biochem Biophys Res Commun 293(1):79-85
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Moye WS, et al.  (1985) Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. J Biol Chem 260(14):8502-8
6) Benjamin PM, et al.  (1989) Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription. Mol Gen Genet 217(2-3):370-7
7) Filetici P, et al.  (1996) Sequence of the GLT1 gene from Saccharomyces cerevisiae reveals the domain structure of yeast glutamate synthase. Yeast 12(13):1359-66