SPC72/YAL047C Summary Help

Standard Name SPC72 1
Systematic Name YAL047C
Alias LDB4
Feature Type ORF, Verified
Description Component of the cytoplasmic Tub4p (gamma-tubulin) complex; binds spindle pole bodies and links them to microtubules; is regulated by Cdc5 kinase; has roles in astral microtubule formation and stabilization (2, 3, 4, 5)
Name Description Spindle Pole Component 1
Chromosomal Location
ChrI:56857 to 54989 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SPC72 GO evidence and references
  View Computational GO annotations for SPC72
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
191 total interaction(s) for 125 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-Western: 14
  • Biochemical Activity: 3
  • Co-localization: 3
  • Co-purification: 1
  • FRET: 2
  • PCA: 6
  • Protein-peptide: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 124

Genetic Interactions
  • Dosage Rescue: 3
  • Synthetic Growth Defect: 9
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 622
Molecular Weight (Da) 72,105
Isoelectric Point (pI) 4.7
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:56857 to 54989 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1869 56857..54989 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000045
References cited on this page View Complete Literature Guide for SPC72
1) Soues S and Adams IR  (1998) SPC72: a spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae. J Cell Sci 111 ( Pt 18)():2809-18
2) Knop M and Schiebel E  (1998) Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation. EMBO J 17(14):3952-67
3) Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77
4) Hoepfner D, et al.  (2002) Reorientation of mispositioned spindles in short astral microtubule mutant spc72Delta is dependent on spindle pole body outer plaque and Kar3 motor protein. Mol Biol Cell 13(4):1366-80
5) Snead JL, et al.  (2007) A Coupled Chemical-Genetic and Bioinformatic Approach to Polo-like Kinase Pathway Exploration. Chem Biol 14(11):1261-72