FUN12/YAL035W Summary Help

Standard Name FUN12 1
Systematic Name YAL035W
Alias yIF2
Feature Type ORF, Verified
Description Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 (2, 3, 4, 5)
Name Description Function Unknown Now 1
Gene Product Alias eIF5B 6
Chromosomal Location
ChrI:76427 to 79435 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All FUN12 GO evidence and references
  View Computational GO annotations for FUN12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
139 total interaction(s) for 85 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 107
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 11
  • Co-crystal Structure: 1
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,002
Molecular Weight (Da) 112,268
Isoelectric Point (pI) 5.55
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:76427 to 79435 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3009 76427..79435 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000033
References cited on this page View Complete Literature Guide for FUN12
1) Coleman KG, et al.  (1986) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome. Mol Cell Biol 6(12):4516-25
2) Choi SK, et al.  (1998) Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast. Science 280(5370):1757-60
3) Lee JH, et al.  (2002) Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation. Proc Natl Acad Sci U S A 99(26):16689-94
4) Lebaron S, et al.  (2012) Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits. Nat Struct Mol Biol 19(8):744-53
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Komar AA, et al.  (2005) Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A. J Biol Chem 280(16):15601-11