Standard Name	FUN12 1
Systematic Name	YAL035W
Alias	yIF2
Feature Type	ORF, Verified
Description	GTPase required for general translation initiation; promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 (2, 3, 4, 5)
Name Description	Function Unknown Now 1
Gene Product Alias	eIF5B 6

Chromosomal Location	ChrI:76427 to 79435 | ORF Map | GBrowse

	View Computational GO annotations for FUN12
Molecular Function
Manually curated	GTP binding (IDA) GTPase activity (IDA, IMP, ISS) ribosome binding (IDA) translation initiation factor activity (IDA)
Biological Process
Manually curated	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) regulation of translational initiation (IMP)
Cellular Component
Manually curated	cytosolic small ribosomal subunit (IDA)
High-throughput	cytoplasm (IDA) mitochondrion (IDA)

Classical genetics
null	20S pre-rRNA accumulation: increased growth in exponential phase: decreased rate inviable vegetative growth: decreased rate viable
Large-scale survey
null	cell cycle progression in G1 phase: increased duration cell size: increased polyphosphate accumulation: abnormal fermentative growth: decreased rate haploinsufficient resistance to hydroxyurea: decreased resistance to camptothecin: decreased resistance to mycophenolic acid: decreased resistance to nickel(2+): decreased resistance to sorbate: decreased resistance to doxorubicin: decreased resistance to L-1,4-dithiothreitol: decreased resistance to sirolimus: decreased resistance to pentachlorophenol: decreased sporulation: absent vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	133 total interaction(s) for 82 unique genes/features.
Physical Interactions	Affinity Capture-MS: 104 Affinity Capture-RNA: 3 Affinity Capture-Western: 11 Co-crystal Structure: 1 Reconstituted Complex: 3 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrI:76427 to 79435 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3009 76427..79435 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000033