VPS8/YAL002W Summary Help

Standard Name VPS8 1, 2
Systematic Name YAL002W
Alias FUN15 3 , VPT8 4 , VPL8 5
Feature Type ORF, Verified
Description Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif (6, 7, 8, 9, 10)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrI:143707 to 147531 | ORF Map | GBrowse
Gene Ontology Annotations All VPS8 GO evidence and references
  View Computational GO annotations for VPS8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 59 genes
Classical genetics
reduction of function
Large-scale survey
544 total interaction(s) for 332 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-Western: 12
  • Reconstituted Complex: 3
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 363
  • Positive Genetic: 81
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 6
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,274
Molecular Weight (Da) 144,961
Isoelectric Point (pI) 5.74
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:143707 to 147531 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2004-01-14
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3825 143707..147531 2011-02-03 2004-01-14
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000002
References cited on this page View Complete Literature Guide for VPS8
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Clark MW, et al.  (1994) Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region. Yeast 10(4):535-41
4) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
6) Horazdovsky BF, et al.  (1996) A novel RING finger protein, Vps8p, functionally interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization. J Biol Chem 271(52):33607-15
7) Luo Wj and Chang A  (1997) Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol 138(4):731-46
8) Chen YJ and Stevens TH  (1996) The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae. Eur J Cell Biol 70(4):289-97
9) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
10) Markgraf DF, et al.  (2009) The CORVET Subunit Vps8 Cooperates with the Rab5 Homolog Vps21 to Induce Clustering of Late Endosomal Compartments. Mol Biol Cell 20(24):5276-89