XRN1/YGL173C Summary Help

Standard Name XRN1 1
Systematic Name YGL173C
Alias DST2 , RAR5 , SEP1 , SKI1 2 , KEM1 3
Feature Type ORF, Verified
Description Evolutionarily-conserved 5'-3' exonuclease; component of cytoplasmic processing (P) bodies involved in mRNA decay; also enters the nucleus and positively regulates transcription initiation and elongation; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p (1, 4, 5, 6, 7, 8, 9, 10)
Name Description eXoRiboNuclease 1
Chromosomal Location
ChrVII:180113 to 175527 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -111 cM
Gene Ontology Annotations All XRN1 GO evidence and references
  View Computational GO annotations for XRN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 97 genes
Classical genetics
reduction of function
Large-scale survey
700 total interaction(s) for 453 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 142
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • Co-purification: 2
  • PCA: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 336
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 23
  • Positive Genetic: 79
  • Synthetic Growth Defect: 43
  • Synthetic Lethality: 32
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 1,528
Molecular Weight (Da) 175,458
Isoelectric Point (pI) 7.5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:180113 to 175527 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -111 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4587 180113..175527 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003141
References cited on this page View Complete Literature Guide for XRN1
1) Larimer FW and Stevens A  (1990) Disruption of the gene XRN1, coding for a 5'----3' exoribonuclease, restricts yeast cell growth. Gene 95(1):85-90
2) Toh-E A, et al.  (1978) Chromosomal superkiller mutants of Saccharomyces cerevisiae. J Bacteriol 136(3):1002-7
3) Kim J, et al.  (1990) kem mutations affect nuclear fusion in Saccharomyces cerevisiae. Genetics 126(4):799-812
4) Solinger JA, et al.  (1999) Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis. Mol Cell Biol 19(9):5930-42
5) Geerlings TH, et al.  (2000) The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5'-->3' exonucleases. RNA 6(12):1698-703
6) Kim J and Kim J  (2002) KEM1 is involved in filamentous growth of Saccharomyces cerevisiae. FEMS Microbiol Lett 216(1):33-8
7) Sheth U and Parker R  (2003) Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science 300(5620):805-8
8) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
9) Sinturel F, et al.  (2012) Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast. Proc Natl Acad Sci U S A 109(21):8264-9
10) Haimovich G, et al.  (2013) Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell 153(5):1000-11