WBP1/YEL002C Summary Help

Standard Name WBP1 1
Systematic Name YEL002C
Feature Type ORF, Verified
Description Beta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum (1, 2, 3 and see Summary Paragraph)
Name Description Wheat germ agglutinin-Binding Protein 1
Chromosomal Location
ChrV:150014 to 148722 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All WBP1 GO evidence and references
  View Computational GO annotations for WBP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
repressible
unspecified
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
494 total interaction(s) for 284 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 26
  • Co-purification: 6
  • PCA: 56
  • Protein-peptide: 3
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Negative Genetic: 320
  • Phenotypic Suppression: 1
  • Positive Genetic: 46
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 430
Molecular Weight (Da) 49,391
Isoelectric Point (pI) 5.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:150014 to 148722 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1293 150014..148722 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000728
SUMMARY PARAGRAPH for WBP1

During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 4, 5).

The oligosaccharyl transferase complex (OST complex) (EC 2.4.1.119) transfers 14-sugar branched oligosaccharides from dolichyl pyrophosphate to asparagine residues. The complex contains nine protein subunits: Ost1p, Ost2p, Ost3p, Ost4p, Ost5p, Ost6p, Stt3p, Swp1p, and Wbp1p, all of which are integral membrane proteins of the ER. The OST complex interacts with the Sec61p pore complex (6) involved in protein import into the ER.

Wbp1p is the beta subunit of the OST complex, one of the original six subunits purified (7). WBP1 is essential (1, 2), but conditional mutants have decreased transferase activity (2, 3). Wbp1p is homologous to mammalian OST48 (OMIM) (8).

Last updated: 2005-06-27 Contact SGD

References cited on this page View Complete Literature Guide for WBP1
1) te Heesen S, et al.  (1991) An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene. Eur J Cell Biol 56(1):8-18
2) te Heesen S, et al.  (1992) The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro. EMBO J 11(6):2071-5
3) te Heesen S, et al.  (1993) Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity. EMBO J 12(1):279-84
4) Herscovics A and Orlean P  (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50
5) Burda P and Aebi M  (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57
6) Chavan MM, et al.  (2005) Subunits of the translocon interact with components of the oligosaccharyl transferase complex. J Biol Chem 280(24):22917-24
7) Kelleher DJ and Gilmore R  (1994) The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides. J Biol Chem 269(17):12908-17
8) Silberstein S, et al.  (1992) The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J Biol Chem 267(33):23658-63