VPS27/YNR006W Summary Help

Standard Name VPS27 1, 2
Systematic Name YNR006W
Alias GRD11 3 , SSV17 , VPL23 4 , VPT27 1 , DID7 5
Feature Type ORF, Verified
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) (6, 7)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXIV:636986 to 638854 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VPS27 GO evidence and references
  View Computational GO annotations for VPS27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All VPS27 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All VPS27 Interaction evidence and references
452 total interaction(s) for 312 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Biochemical Activity: 14
  • Co-crystal Structure: 2
  • Co-localization: 1
  • PCA: 3
  • Protein-peptide: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 8
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Negative Genetic: 303
  • Phenotypic Enhancement: 36
  • Phenotypic Suppression: 8
  • Positive Genetic: 21
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4
Resources
Expression Summary
histogram
Resources
Protein Information All VPS27 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:636986 to 638854 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1869 636986..638854 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005289
References cited on this page View Complete Literature Guide for VPS27
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Nothwehr SF, et al.  (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
6) Piper RC, et al.  (1995) VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J Cell Biol 131(3):603-17
7) Bilodeau PS, et al.  (2002) The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nat Cell Biol 4(7):534-9