Standard Name	VTC4 1
Systematic Name	YJL012C
Alias	PHM3 2 , YJL012C-A
Feature Type	ORF, Verified
Description	Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description	Vacuolar Transporter Chaperone 1

Chromosomal Location	ChrX:413399 to 411234 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for VTC4
Molecular Function
Manually curated	polyphosphate kinase activity (IDA)
Biological Process
Manually curated	microautophagy (IDA, IMP) polyphosphate metabolic process (IMP) vacuolar transport (IDA, IMP) vacuole fusion, non-autophagic (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) intrinsic to vacuolar membrane (IDA) vacuolar transporter chaperone complex (IPI)
High-throughput	integral to membrane (ISM)

Classical genetics
null	polyphosphate accumulation: absent phosphate(3-) accumulation: decreased rate inviable metal resistance: decreased metal resistance: increased resistance to sirolimus: normal small molecule transport: decreased rate vegetative growth: arrested
overexpression	resistance to nocodazole: decreased resistance to hydroxyurea: decreased
Large-scale survey
null	polyphosphate accumulation: abnormal competitive fitness: decreased dessication resistance: decreased metal resistance: increased resistance to streptomycin: decreased resistance to paromomycin: decreased resistance to cycloheximide: decreased resistance to nickel(2+): increased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to ciclopirox olamine: decreased
overexpression	cell cycle progression: abnormal
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	56 total interaction(s) for 41 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 2 Affinity Capture-Western: 4 PCA: 8 Reconstituted Complex: 1 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 7 Phenotypic Enhancement: 2 Phenotypic Suppression: 12 Positive Genetic: 4 Synthetic Growth Defect: 4 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrX:413399 to 411234 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	This feature contains embedded feature(s): YJL012C-A
Last Update	Coordinates: 2011-02-03 | Sequence: 2004-02-19
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2166 413399..411234 2011-02-03 2004-02-19
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003549