VRG4/YGL225W Summary Help

Standard Name VRG4 1, 2
Systematic Name YGL225W
Alias VAN2 3 , GOG5 , LDB3 , VIG4 4
Feature Type ORF, Verified
Description Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication (1, 2, 5, 6)
Name Description Vandate Resistance Glycosylation 1
Chromosomal Location
ChrVII:76894 to 77907 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VRG4 GO evidence and references
  View Computational GO annotations for VRG4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
unspecified
Large-scale survey
conditional
null
reduction of function
repressible
Resources
74 total interaction(s) for 47 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Co-purification: 2
  • PCA: 1
  • Two-hybrid: 6

Genetic Interactions
  • Negative Genetic: 57
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 337
Molecular Weight (Da) 37,018
Isoelectric Point (pI) 10.08
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:76894 to 77907 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1014 76894..77907 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000003193
References cited on this page View Complete Literature Guide for VRG4
1) Ballou L, et al.  (1991) Vanadate-resistant yeast mutants are defective in protein glycosylation. Proc Natl Acad Sci U S A 88(8):3209-12
2) Poster JB and Dean N  (1996) The yeast VRG4 gene is required for normal Golgi functions and defines a new family of related genes. J Biol Chem 271(7):3837-45
3) Kanik-Ennulat C and Neff N  (1990) Vanadate-resistant mutants of Saccharomyces cerevisiae show alterations in protein phosphorylation and growth control. Mol Cell Biol 10(3):898-909
4) Abe M, et al.  (1999) Molecular characterization of Vig4/Vrg4 GDP-mannose transporter of the yeast Saccharomyces cerevisiae. FEBS Lett 458(3):309-12
5) Dean N, et al.  (1997) The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae. J Biol Chem 272(50):31908-14
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61