VPS9/YML097C Summary Help

Standard Name VPS9 1, 2, 3
Systematic Name YML097C
Alias VPL31 4 , VPT9 1
Feature Type ORF, Verified
Description Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; binds ubiquitin (3, 5, 6, 7)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXIII:79690 to 78335 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS9 GO evidence and references
  View Computational GO annotations for VPS9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All VPS9 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
interactions All VPS9 Interaction evidence and references
398 total interaction(s) for 247 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 245
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 54
  • Positive Genetic: 35
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 1
Resources
Expression Summary
histogram
Resources
Protein Information All VPS9 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:79690 to 78335 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1356 79690..78335 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004563
References cited on this page View Complete Literature Guide for VPS9
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Burd CG, et al.  (1996) A yeast protein related to a mammalian Ras-binding protein, Vps9p, is required for localization of vacuolar proteins. Mol Cell Biol 16(5):2369-77
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Hama H, et al.  (1999) Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport. J Biol Chem 274(21):15284-91
6) Davies BA, et al.  (2003) Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. J Biol Chem 278(22):19826-33
7) Cabrera M, et al.  (2013) Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem 288(7):5166-75