VPS8/YAL002W Summary Help

Standard Name VPS8 1, 2
Systematic Name YAL002W
Alias FUN15 3 , VPT8 4 , VPL8 5
Feature Type ORF, Verified
Description Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif (6, 7, 8, 9, 10)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrI:143707 to 147531 | ORF Map | GBrowse
Gene Ontology Annotations All VPS8 GO evidence and references
  View Computational GO annotations for VPS8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
549 total interaction(s) for 335 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-Western: 12
  • Reconstituted Complex: 3
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 365
  • Phenotypic Enhancement: 2
  • Positive Genetic: 81
  • Synthetic Growth Defect: 34
  • Synthetic Lethality: 6
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,274
Molecular Weight (Da) 144,961
Isoelectric Point (pI) 5.74
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:143707 to 147531 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2004-01-14
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3825 143707..147531 2011-02-03 2004-01-14
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000002
References cited on this page View Complete Literature Guide for VPS8
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Clark MW, et al.  (1994) Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region. Yeast 10(4):535-41
4) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
6) Horazdovsky BF, et al.  (1996) A novel RING finger protein, Vps8p, functionally interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization. J Biol Chem 271(52):33607-15
7) Luo Wj and Chang A  (1997) Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol 138(4):731-46
8) Chen YJ and Stevens TH  (1996) The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae. Eur J Cell Biol 70(4):289-97
9) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
10) Markgraf DF, et al.  (2009) The CORVET Subunit Vps8 Cooperates with the Rab5 Homolog Vps21 to Induce Clustering of Late Endosomal Compartments. Mol Biol Cell 20(24):5276-89