VPS75/YNL246W Summary Help

Standard Name VPS75 1
Systematic Name YNL246W
Feature Type ORF, Verified
Description NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Vacuolar Protein Sorting 1
Chromosomal Location
ChrXIV:185460 to 186349 | ORF Map | GBrowse
Gene Ontology Annotations All VPS75 GO evidence and references
  View Computational GO annotations for VPS75
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
132 total interaction(s) for 96 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 8
  • Biochemical Activity: 4
  • Co-crystal Structure: 8
  • Co-purification: 1
  • Reconstituted Complex: 19
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 37
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 4
  • Positive Genetic: 16
  • Synthetic Growth Defect: 15
  • Synthetic Haploinsufficiency: 1

Expression Summary
Length (a.a.) 264
Molecular Weight (Da) 30,615
Isoelectric Point (pI) 4.47
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:185460 to 186349 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..32 185460..185491 2011-02-03 1996-07-31
Intron 33..127 185492..185586 2011-02-03 1996-07-31
CDS 128..890 185587..186349 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005190
References cited on this page View Complete Literature Guide for VPS75
1) Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501
2) Selth L and Svejstrup JQ  (2007) Vps75, A New Yeast Member of the NAP Histone Chaperone Family. J Biol Chem 282(17):12358-62
3) Han J, et al.  (2007) The Rtt109-Vps75 Histone Acetyltransferase Complex Acetylates Non-nucleosomal Histone H3. J Biol Chem 282(19):14158-64
4) Jessulat M, et al.  (2008) Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys 469(2):157-64
5) Fillingham J, et al.  (2008) Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109. Mol Cell Biol 28(13):4342-53
6) Park YJ, et al.  (2008) Histone chaperone specificity in Rtt109 activation. Nat Struct Mol Biol 15(9):957-64
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30