VPS74/YDR372C Summary Help

Standard Name VPS74 1
Systematic Name YDR372C
Alias API1 2 , MNN3 3 , 4
Feature Type ORF, Verified
Description Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 (5, 6, 7, 8, 9)
Name Description Vacuolar Protein Sorting 1
Chromosomal Location
ChrIV:1222149 to 1221112 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All VPS74 GO evidence and references
  View Computational GO annotations for VPS74
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
217 total interaction(s) for 149 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Far Western: 1
  • PCA: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 95
  • Phenotypic Enhancement: 34
  • Phenotypic Suppression: 7
  • Positive Genetic: 7
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 345
Molecular Weight (Da) 39,286
Isoelectric Point (pI) 4.85
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1222149 to 1221112 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1038 1222149..1221112 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002780
References cited on this page View Complete Literature Guide for VPS74
1) Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501
2) Bidlingmaier S and Snyder M  (2002) Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics 1(6):345-56
3) Raschke WC, et al.  (1973) Genetic control of yeast mannan structure. Isolation and characterization of mannan mutants. J Biol Chem 248(13):4660-6
4) Corbacho I, et al.  (2010) Identification of the MNN3 gene of Saccharomyces cerevisiae. Glycobiology 20(11):1336-40
5) Schmitz KR, et al.  (2008) Golgi localization of glycosyltransferases requires a Vps74p oligomer. Dev Cell 14(4):523-34
6) Dippold HC, et al.  (2009) GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote budding. Cell 139(2):337-51
7) Wood CS, et al.  (2009) PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi trafficking. J Cell Biol 187(7):967-75
8) Wood CS, et al.  (2012) Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase. Mol Biol Cell 23(13):2527-36
9) Wu CC, et al.  (2000) GMx33: a novel family of trans-Golgi proteins identified by proteomics. Traffic 1(12):963-75