VPS64/YDR200C Summary Help

Standard Name VPS64 1
Systematic Name YDR200C
Alias FAR9 2
Feature Type ORF, Verified
Description Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Vacuolar Protein Sorting 1
Chromosomal Location
ChrIV:856093 to 854279 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS64 GO evidence and references
  View Computational GO annotations for VPS64
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
200 total interaction(s) for 170 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • PCA: 2
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 124
  • Phenotypic Enhancement: 1
  • Positive Genetic: 24
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 5
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 604
Molecular Weight (Da) 67,283
Isoelectric Point (pI) 7.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:856093 to 854279 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1815 856093..854279 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002608
References cited on this page View Complete Literature Guide for VPS64
1) Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501
2) Kemp HA and Sprague Jr GF Jr  (2003) Far3 and five interacting proteins prevent premature recovery from pheromone arrest in the budding yeast Saccharomyces cerevisiae. Mol Cell Biol 23(5):1750-63
3) Beilharz T, et al.  (2003) Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae. J Biol Chem 278(10):8219-23
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83