VPS60/YDR486C Summary Help

Standard Name VPS60 1
Systematic Name YDR486C
Alias MOS10 2 , CHM5 3
Feature Type ORF, Verified
Description Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p (1, 4, 5)
Name Description Vacuolar Protein Sorting 1
Chromosomal Location
ChrIV:1428120 to 1427431 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS60 GO evidence and references
  View Computational GO annotations for VPS60
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
137 total interaction(s) for 115 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • PCA: 6
  • Reconstituted Complex: 2
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 73
  • Phenotypic Enhancement: 4
  • Positive Genetic: 7
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 229
Molecular Weight (Da) 25,861
Isoelectric Point (pI) 4.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1428120 to 1427431 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..690 1428120..1427431 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002894
References cited on this page View Complete Literature Guide for VPS60
1) Kohler JR  (2003) Mos10 (Vps60) is required for normal filament maturation in Saccharomyces cerevisiae. Mol Microbiol 49(5):1267-85
2) Kranz A, et al.  (2001) A family of small coiled-coil-forming proteins functioning at the late endosome in yeast. Mol Biol Cell 12(3):711-23
3) Howard TL, et al.  (2001) CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci 114(Pt 13):2395-404
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Rue SM, et al.  (2008) Novel ist1-did2 complex functions at a late step in multivesicular body sorting. Mol Biol Cell 19(2):475-84