VPS54/YDR027C Summary Help

Standard Name VPS54 1
Systematic Name YDR027C
Alias LUV1 , CGP1 , TCS3
Feature Type ORF, Verified
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentially phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p (2, 3, 4)
Name Description Vacuolar Protein Sorting
Chromosomal Location
ChrIV:497318 to 494649 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS54 GO evidence and references
  View Computational GO annotations for VPS54
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
153 total interaction(s) for 93 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-Western: 19
  • Biochemical Activity: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 22
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 28
  • Positive Genetic: 4
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 889
Molecular Weight (Da) 101,519
Isoelectric Point (pI) 6.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:497318 to 494649 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2670 497318..494649 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002434
References cited on this page View Complete Literature Guide for VPS54
1) Conibear E and Stevens TH  (2000) Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell 11(1):305-23
2) Conibear E, et al.  (2003) Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell 14(4):1610-23
3) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4) Dotiwala F, et al.  (2013) DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase. Proc Natl Acad Sci U S A 110(1):E41-9