Standard Name	VPS52 1, 2
Systematic Name	YDR484W
Alias	SAC2 3
Feature Type	ORF, Verified
Description	Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin (1, 4, 5)
Name Description	Vacuolar Protein Sorting

Chromosomal Location	ChrIV:1422763 to 1424688 | ORF Map | GBrowse
	Genetic position: 314 cM

	View Computational GO annotations for VPS52
Molecular Function
Manually curated	protein binding (IPI)
Biological Process
Manually curated	actin filament-based process (IGI) Golgi to vacuole transport (IMP) retrograde transport, endosome to Golgi (IDA)
Cellular Component
Manually curated	GARP complex (IPI) Golgi apparatus (IDA, TAS)

Classical genetics
null	acid pH resistance: decreased actin cytoskeleton morphology: abnormal alkaline pH resistance: decreased autophagy: decreased autophagy: abnormal lithium(1+) accumulation: increased ionic stress resistance: decreased meiosis: abnormal metal resistance: decreased mitochondrial morphology: abnormal alpha-factor secretion: decreased carboxypeptidase Y secretion: decreased GFP-Snc1p distribution: abnormal Ape1p (API) modification: normal Ape1p (API) modification: absent resistance to cobalt chloride hexahydrate: decreased resistance to monensin: decreased resistance to caffeine: decreased resistance to sirolimus: decreased resistance to gentamycin: decreased resistance to ferrozine(2-): decreased sporulation efficiency: abnormal vacuolar morphology: abnormal viability: decreased
Large-scale survey
null	acid pH resistance: decreased anaerobic growth: decreased proton accumulation: increased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased ionic stress resistance: decreased metal resistance: decreased Can1p-GFP distribution: abnormal resistance to calcium dichloride: decreased resistance to curcumin: decreased resistance to BPS: decreased resistance to wortmannin: normal resistance to ciclopirox olamine: decreased resistance to mefloquine: decreased resistance to chloroquine: decreased resistance to spermine: decreased resistance to hygromycin B: decreased resistance to neothyonidioside: decreased resistance to quinine: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to wortmannin: decreased resistance to fluconazole: decreased resistance to caffeine: decreased resistance to dihydromotuporamine C: decreased resistance to monensin: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to sulfanilamide: decreased resistance to sirolimus: decreased resistance to caspofungin: increased resistance to cycloheximide: decreased resistance to amitrole: decreased respiratory growth: decreased rate sporulation: decreased stress resistance: decreased toxin resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	233 total interaction(s) for 126 unique genes/features.
Physical Interactions	Affinity Capture-MS: 24 Affinity Capture-RNA: 1 Affinity Capture-Western: 16 Co-fractionation: 1 PCA: 1 Reconstituted Complex: 1 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 85 Phenotypic Enhancement: 20 Phenotypic Suppression: 40 Positive Genetic: 15 Synthetic Growth Defect: 8 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 12 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:1422763 to 1424688 | ORF Map | GBrowse
	Genetic position: 314 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1926 1422763..1424688 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002892