VPS45/YGL095C Summary Help

Standard Name VPS45 1
Systematic Name YGL095C
Alias STT10 2 , VPL28 1
Feature Type ORF, Verified
Description Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment (3, 4, 5)
Name Description Vacuolar Protein Sorting 1
Chromosomal Location
ChrVII:330607 to 328874 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All VPS45 GO evidence and references
  View Computational GO annotations for VPS45
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
89 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 7
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 19
  • Phenotypic Enhancement: 8
  • Positive Genetic: 1
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 7

Expression Summary
Length (a.a.) 577
Molecular Weight (Da) 67,017
Isoelectric Point (pI) 6.2
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:330607 to 328874 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1734 330607..328874 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003063
References cited on this page View Complete Literature Guide for VPS45
1) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
2) Yoshida S, et al.  (1995) STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway. Gene 160(1):117-22
3) Piper RC, et al.  (1994) Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles. Eur J Cell Biol 65(2):305-18
4) Bryant NJ, et al.  (1998) Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: a VPS45-dependent intracellular route and a VPS45-independent, endocytic route. Eur J Cell Biol 76(1):43-52
5) Bryant NJ and James DE  (2001) Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation. EMBO J 20(13):3380-8