VPS29/YHR012W Summary Help

Standard Name VPS29 1, 2, 3
Systematic Name YHR012W
Alias PEP11 , VPT6 4
Feature Type ORF, Verified
Description Subunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval (5, 6, 7)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrVIII:129481 to 130448 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VPS29 GO evidence and references
  View Computational GO annotations for VPS29
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
overexpression
Resources
271 total interaction(s) for 155 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 13
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • Co-purification: 2
  • PCA: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 138
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 11
  • Positive Genetic: 23
  • Synthetic Growth Defect: 27
  • Synthetic Lethality: 9
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 282
Molecular Weight (Da) 31,012
Isoelectric Point (pI) 4.37
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:129481 to 130448 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..48 129481..129528 2011-02-03 1996-07-31
Intron 49..167 129529..129647 2011-02-03 1996-07-31
CDS 168..968 129648..130448 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001054
References cited on this page View Complete Literature Guide for VPS29
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Friedrichsen, D.M., et al.  (1995) Analysis of the PEP11 locus in Saccharomyces cerevisiae. Unpublished
4) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5) Seaman MN, et al.  (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
6) Seaman MN, et al.  (1997) Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products. J Cell Biol 137(1):79-92
7) Reddy JV and Seaman MN  (2001) Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell 12(10):3242-56