VPS29/YHR012W Summary

VPS29/YHR012W Summary

Standard Name	VPS29 1, 2, 3
Systematic Name	YHR012W
Alias	PEP11 , VPT6 4
Feature Type	ORF, Verified
Description	Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval (5, 6, 7)
Name Description	Vacuolar Protein Sorting 1, 2

Chromosomal Location	ChrVIII:129481 to 130448 \| ORF Map \| GBrowse

Gene Ontology Annotations All VPS29 GO evidence and references

	View Computational GO annotations for VPS29
Molecular Function
Manually curated	contributes_to protein transporter activity (IMP, IPI)
Biological Process
Manually curated	retrograde transport, endosome to Golgi (IPI)
Cellular Component
Manually curated	endosome (IPI) retromer complex (IMP, IPI) retromer complex, inner shell (IPI)

Mutant phenotypes All VPS29 Phenotype evidence and references

Classical genetics
null	vacuolar transport: abnormal viability: decreased
reduction of function	vacuolar transport: abnormal
unspecified	carboxypeptidase-Y-invertase fusion protein distribution: abnormal
Large-scale survey
null	glycogen accumulation: increased rubidium(1+) accumulation: decreased glutathione excretion: increased inositol excretion: increased chitin deposition: normal competitive fitness: decreased dessication resistance: decreased Cps1p (CPS) modification: decreased Pho8p (ALP) modification: decreased resistance to caffeine: decreased resistance to ethanol: decreased resistance to nickel sulfate: increased resistance to cisplatin: increased resistance to wortmannin: increased resistance to fluconazole: decreased resistance to sirolimus: decreased resistance to (S)-lactic acid: decreased resistance to hydrogen chloride: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to sodium selenide: decreased resistance to dimethyl sulfoxide: decreased resistance to mefloquine: decreased resistance to methyl methanesulfonate: decreased resistance to benomyl: decreased stress resistance: decreased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All VPS29 Interaction evidence and references

	265 total interaction(s) for 151 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 2 Affinity Capture-Western: 13 Biochemical Activity: 1 Co-fractionation: 2 Co-purification: 2 PCA: 4
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 137 Phenotypic Enhancement: 15 Phenotypic Suppression: 11 Positive Genetic: 23 Synthetic Growth Defect: 26 Synthetic Lethality: 9 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All VPS29 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVIII:129481 to 130448 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..48	129481..129528	2011-02-03	1996-07-31
Intron	49..167	129529..129647	2011-02-03	1996-07-31
CDS	168..968	129648..130448	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001054

References cited on this page View Complete Literature Guide for VPS29

1)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3)	Friedrichsen, D.M., et al. (1995) Analysis of the PEP11 locus in Saccharomyces cerevisiae. Unpublished
4)	Bankaitis VA, et al. (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5)	Seaman MN, et al. (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
6)	Seaman MN, et al. (1997) Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products. J Cell Biol 137(1):79-92
7)	Reddy JV and Seaman MN (2001) Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell 12(10):3242-56