VPS28/YPL065W Summary Help

Standard Name VPS28 1, 2
Systematic Name YPL065W
Alias VPT28 1 , VPL13 2
Feature Type ORF, Verified
Description Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p (3, 4)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXVI:427927 to 428655 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VPS28 GO evidence and references
  View Computational GO annotations for VPS28
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
110 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 8
  • Co-crystal Structure: 2
  • Co-fractionation: 2
  • Co-purification: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 18
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 27
  • Positive Genetic: 3
  • Synthetic Growth Defect: 4
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 242
Molecular Weight (Da) 27,702
Isoelectric Point (pI) 5.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:427927 to 428655 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..729 427927..428655 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005986
References cited on this page View Complete Literature Guide for VPS28
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Katzmann DJ, et al.  (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55
4) Pineda-Molina E, et al.  (2006) The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment. Traffic 7(8):1007-16