VPS16/YPL045W Summary Help

Standard Name VPS16 1, 2
Systematic Name YPL045W
Alias SVL6 3 , VAM9 , VPT16 1
Feature Type ORF, Verified
Description Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages (4, 5, 6)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXVI:467260 to 469656 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VPS16 GO evidence and references
  View Computational GO annotations for VPS16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All VPS16 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
interactions All VPS16 Interaction evidence and references
115 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-Western: 36
  • Co-fractionation: 1
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 9
Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 8
  • Phenotypic Enhancement: 4
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 5
Resources
Expression Summary
histogram
Resources
Protein Information All VPS16 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:467260 to 469656 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2397 467260..469656 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005966
References cited on this page View Complete Literature Guide for VPS16
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Zheng B, et al.  (1998) Isolation of yeast mutants defective for localization of vacuolar vital dyes. Proc Natl Acad Sci U S A 95(20):11721-6
4) Seals DF, et al.  (2000) A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion. Proc Natl Acad Sci U S A 97(17):9402-7
5) Rieder SE and Emr SD  (1997) A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. Mol Biol Cell 8(11):2307-27
6) Peterson MR and Emr SD  (2001) The class C Vps complex functions at multiple stages of the vacuolar transport pathway. Traffic 2(7):476-86