VPS15/YBR097W Summary Help

Standard Name VPS15 1, 2
Systematic Name YBR097W
Alias GRD8 , VAC4 , VPL19 2 , VPS40 3 , VPT15 4
Feature Type ORF, Verified
Description Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery (5, 6, 7, 8, 9, 10)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrII:436951 to 441315 | ORF Map | GBrowse
Gene Ontology Annotations All VPS15 GO evidence and references
  View Computational GO annotations for VPS15
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
43 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-Western: 9
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 6

Expression Summary
Length (a.a.) 1,454
Molecular Weight (Da) 166,372
Isoelectric Point (pI) 6.76
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:436951 to 441315 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4365 436951..441315 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000301
References cited on this page View Complete Literature Guide for VPS15
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
4) Seeley ES, et al.  (2002) Genomic analysis of homotypic vacuole fusion. Mol Biol Cell 13(3):782-94
5) Stack JH and Emr SD  (1994) Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269(50):31552-62
6) Stack JH, et al.  (1995) Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins. Annu Rev Cell Dev Biol 11():1-33
7) Stack JH, et al.  (1995) Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J Cell Biol 129(2):321-34
8) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
9) Slessareva JE, et al.  (2006) Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome. Cell 126(1):191-203
10) Gaur NA, et al.  (2013) Vps factors are required for efficient transcription elongation in budding yeast. Genetics 193(3):829-51