VPS1/YKR001C Summary Help

Standard Name VPS1 1, 2, 3
Systematic Name YKR001C
Alias GRD1 , LAM1 , SPO15 , VPL1 4 , VPT26 1
Feature Type ORF, Verified
Description Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis (5, 6, 7, 8, 9)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXI:442722 to 440608 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -1 cM
Gene Ontology Annotations All VPS1 GO evidence and references
  View Computational GO annotations for VPS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
686 total interaction(s) for 447 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 72
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Co-fractionation: 1
  • Far Western: 1
  • FRET: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 370
  • Phenotypic Enhancement: 21
  • Phenotypic Suppression: 104
  • Positive Genetic: 32
  • Synthetic Growth Defect: 30
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 25
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 704
Molecular Weight (Da) 78,736
Isoelectric Point (pI) 8.15
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:442722 to 440608 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2115 442722..440608 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001709
References cited on this page View Complete Literature Guide for VPS1
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Bloom, K.  (1992) Personal Communication, Mortimer Map Edition 11
4) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
5) Ekena K, et al.  (1993) The VPS1 protein is a dynamin-like GTPase required for sorting proteins to the yeast vacuole. Ciba Found Symp 176():198-211; discussion 211-4
6) Nothwehr SF, et al.  (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
7) Vizeacoumar FJ, et al.  (2003) YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae. J Cell Biol 161(2):321-32
8) Yu X and Cai M  (2004) The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p. J Cell Sci 117(Pt 17):3839-53
9) Rooij II, et al.  (2010) A role for the dynamin-like protein Vps1 during endocytosis in yeast. J Cell Sci 123(Pt 20):3496-506