VPH2/YKL119C Summary Help

Standard Name VPH2
Systematic Name YKL119C
Alias CLS10 , VMA12
Feature Type ORF, Verified
Description Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) (1, 2, 3, 4)
Name Description Vacuolar pH
Chromosomal Location
ChrXI:219217 to 218570 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPH2 GO evidence and references
  View Computational GO annotations for VPH2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All VPH2 Phenotype evidence and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
interactions All VPH2 Interaction evidence and references
282 total interaction(s) for 219 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • PCA: 2
Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 197
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 51
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 3
Resources
Expression Summary
histogram
Resources
Protein Information All VPH2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:219217 to 218570 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..648 219217..218570 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001602
References cited on this page View Complete Literature Guide for VPH2
1) Preston RA, et al.  (1992) Genes required for vacuolar acidity in Saccharomyces cerevisiae. Genetics 131(3):551-8
2) Jackson DD and Stevens TH  (1997) VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly. J Biol Chem 272(41):25928-34
3) Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4
4) Graham LA and Stevens TH  (1999) Assembly of the yeast vacuolar proton-translocating ATPase. J Bioenerg Biomembr 31(1):39-47