VMA6/YLR447C Summary Help

Standard Name VMA6
Systematic Name YLR447C
Feature Type ORF, Verified
Description Subunit d of the V0 integral membrane domain of V-ATPase; part of the electrogenic proton pump found in the endomembrane system; required for V1 domain assembly on the vacuolar membrane; the V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase) has five subunits (1, 2 and see Summary Paragraph)
Chromosomal Location
ChrXII:1027893 to 1026856 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VMA6 GO evidence and references
  View Computational GO annotations for VMA6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
94 total interaction(s) for 64 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 31
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 7
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 7
  • Protein-peptide: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 7
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 345
Molecular Weight (Da) 39,790
Isoelectric Point (pI) 4.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:1027893 to 1026856 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1038 1027893..1026856 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004439
SUMMARY PARAGRAPH for VMA6

VMA6 encodes the d subunit of the yeast V-ATPase V0 domain (1). Vacuolar (H )-ATPases (V-ATPases) are ATP-dependent proton pumps that acidify intracellular vacuolar compartments. Vacuolar acidification is important for many cellular processes, including endocytosis, targeting of newly synthesized lysosomal enzymes, and other molecular targeting processes. The V-ATPase consists of two separable domains. The V1 domain has eight known subunits, is peripherally associated with the vacuolar membrane, and catalyzes ATP hydrolysis. The V0 domain is an integral membrane structure of five subunits, and transports protons across the membrane. The structure, function, and assembly of V-ATPases are reviewed in references 2, 3, 4 and 5.

The vma6 null mutant is viable but shows a growth defect at neutral pH and is sensitive to calcium (1). In the absence of Vma6p, the remaining V0 subunits are destabilized and the V1 domain does not assemble onto the vacuolar membrane (1).

V-ATPases have been identified in numerous eukaryotes (2, 5); Vma6p homologs have been identified in red beet and Arabidopsis (6).

Last updated: 2000-05-17 Contact SGD

References cited on this page View Complete Literature Guide for VMA6
1) Bauerle C, et al.  (1993) The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector. J Biol Chem 268(17):12749-57
2) Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4
3) Graham LA and Stevens TH  (1999) Assembly of the yeast vacuolar proton-translocating ATPase. J Bioenerg Biomembr 31(1):39-47
4) Kane PM  (1999) Biosynthesis and regulation of the yeast vacuolar H+-ATPase. J Bioenerg Biomembr 31(1):49-56
5) Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808
6) Bauerle C, et al.  (1998) Characterization of a red beet protein homologous to the essential 36-kilodalton subunit of the yeast V-type ATPase. Plant Physiol 117(3):859-67