VMA22/YHR060W Summary Help

Standard Name VMA22
Systematic Name YHR060W
Alias CEV1 1 , VPH6
Feature Type ORF, Verified
Description Protein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) (2, 3, 4)
Chromosomal Location
ChrVIII:220725 to 221270 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VMA22 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
56 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • PCA: 2
  • Two-hybrid: 8

Genetic Interactions
  • Negative Genetic: 20
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 181
Molecular Weight (Da) 21,078
Isoelectric Point (pI) 5.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:220725 to 221270 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..546 220725..221270 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001102
References cited on this page View Complete Literature Guide for VMA22
1) Hemenway CS, et al.  (1995) vph6 mutants of Saccharomyces cerevisiae require calcineurin for growth and are defective in vacuolar H(+)-ATPase assembly. Genetics 141(3):833-44
2) Graham LA, et al.  (1998) Assembly of the yeast vacuolar H+-ATPase occurs in the endoplasmic reticulum and requires a Vma12p/Vma22p assembly complex. J Cell Biol 142(1):39-49
3) Graham LA and Stevens TH  (1999) Assembly of the yeast vacuolar proton-translocating ATPase. J Bioenerg Biomembr 31(1):39-47
4) Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808